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p aeruginosa pao1  (ATCC)
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ATCC p aeruginosa pao1
( a ) Colonies without MnCl₂ exhibited rapid expansion and increased margin irregularity, highlighting dynamic interactions within the microbial community. ( b ) Mn(II) oxidation rates by KT2440, assessed across various inoculum densities in co-culture with <t>PAO1</t> and in monoculture (n = 3), showed significant differences at lower densities. ( c ) Following MnCl₂ addition, KT2440 demonstrated significantly enhanced survival at lower inoculum densities (n = 6), accompanied by visible brown Mn oxides after 12 h, suggesting a protective role of these oxides. Significance (* p < 0.05) was assessed using the Wilcoxon test. ( d ) The relationship between survival and incremental Mn(II) oxidation rate in KT2440. Equation depicted the relationship, while R 2 indicated the linear correlation between survival and Mn(II) oxidation rate. In all regression analyses, the T-test yielded p -values less than 0.05.
P Aeruginosa Pao1, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 8  (Sino Biological)
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Sino Biological il 8
( a ) Colonies without MnCl₂ exhibited rapid expansion and increased margin irregularity, highlighting dynamic interactions within the microbial community. ( b ) Mn(II) oxidation rates by KT2440, assessed across various inoculum densities in co-culture with <t>PAO1</t> and in monoculture (n = 3), showed significant differences at lower densities. ( c ) Following MnCl₂ addition, KT2440 demonstrated significantly enhanced survival at lower inoculum densities (n = 6), accompanied by visible brown Mn oxides after 12 h, suggesting a protective role of these oxides. Significance (* p < 0.05) was assessed using the Wilcoxon test. ( d ) The relationship between survival and incremental Mn(II) oxidation rate in KT2440. Equation depicted the relationship, while R 2 indicated the linear correlation between survival and Mn(II) oxidation rate. In all regression analyses, the T-test yielded p -values less than 0.05.
Il 8, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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10098 2 ap  (Proteintech)
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Proteintech 10098 2 ap
( a ) Colonies without MnCl₂ exhibited rapid expansion and increased margin irregularity, highlighting dynamic interactions within the microbial community. ( b ) Mn(II) oxidation rates by KT2440, assessed across various inoculum densities in co-culture with <t>PAO1</t> and in monoculture (n = 3), showed significant differences at lower densities. ( c ) Following MnCl₂ addition, KT2440 demonstrated significantly enhanced survival at lower inoculum densities (n = 6), accompanied by visible brown Mn oxides after 12 h, suggesting a protective role of these oxides. Significance (* p < 0.05) was assessed using the Wilcoxon test. ( d ) The relationship between survival and incremental Mn(II) oxidation rate in KT2440. Equation depicted the relationship, while R 2 indicated the linear correlation between survival and Mn(II) oxidation rate. In all regression analyses, the T-test yielded p -values less than 0.05.
10098 2 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human il-8 / cxcl8 protein  (Sino Biological)
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Sino Biological human il-8 / cxcl8 protein
( a ) Colonies without MnCl₂ exhibited rapid expansion and increased margin irregularity, highlighting dynamic interactions within the microbial community. ( b ) Mn(II) oxidation rates by KT2440, assessed across various inoculum densities in co-culture with <t>PAO1</t> and in monoculture (n = 3), showed significant differences at lower densities. ( c ) Following MnCl₂ addition, KT2440 demonstrated significantly enhanced survival at lower inoculum densities (n = 6), accompanied by visible brown Mn oxides after 12 h, suggesting a protective role of these oxides. Significance (* p < 0.05) was assessed using the Wilcoxon test. ( d ) The relationship between survival and incremental Mn(II) oxidation rate in KT2440. Equation depicted the relationship, while R 2 indicated the linear correlation between survival and Mn(II) oxidation rate. In all regression analyses, the T-test yielded p -values less than 0.05.
Human Il 8 / Cxcl8 Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human il 6  (Sino Biological)
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Sino Biological recombinant human il 6
( a ) Colonies without MnCl₂ exhibited rapid expansion and increased margin irregularity, highlighting dynamic interactions within the microbial community. ( b ) Mn(II) oxidation rates by KT2440, assessed across various inoculum densities in co-culture with <t>PAO1</t> and in monoculture (n = 3), showed significant differences at lower densities. ( c ) Following MnCl₂ addition, KT2440 demonstrated significantly enhanced survival at lower inoculum densities (n = 6), accompanied by visible brown Mn oxides after 12 h, suggesting a protective role of these oxides. Significance (* p < 0.05) was assessed using the Wilcoxon test. ( d ) The relationship between survival and incremental Mn(II) oxidation rate in KT2440. Equation depicted the relationship, while R 2 indicated the linear correlation between survival and Mn(II) oxidation rate. In all regression analyses, the T-test yielded p -values less than 0.05.
Recombinant Human Il 6, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sites 32 60  (ATCC)
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ATCC sites 32 60
( a ) Colonies without MnCl₂ exhibited rapid expansion and increased margin irregularity, highlighting dynamic interactions within the microbial community. ( b ) Mn(II) oxidation rates by KT2440, assessed across various inoculum densities in co-culture with <t>PAO1</t> and in monoculture (n = 3), showed significant differences at lower densities. ( c ) Following MnCl₂ addition, KT2440 demonstrated significantly enhanced survival at lower inoculum densities (n = 6), accompanied by visible brown Mn oxides after 12 h, suggesting a protective role of these oxides. Significance (* p < 0.05) was assessed using the Wilcoxon test. ( d ) The relationship between survival and incremental Mn(II) oxidation rate in KT2440. Equation depicted the relationship, while R 2 indicated the linear correlation between survival and Mn(II) oxidation rate. In all regression analyses, the T-test yielded p -values less than 0.05.
Sites 32 60, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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daptomycin  (ATCC)
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ATCC daptomycin
( a ) Colonies without MnCl₂ exhibited rapid expansion and increased margin irregularity, highlighting dynamic interactions within the microbial community. ( b ) Mn(II) oxidation rates by KT2440, assessed across various inoculum densities in co-culture with <t>PAO1</t> and in monoculture (n = 3), showed significant differences at lower densities. ( c ) Following MnCl₂ addition, KT2440 demonstrated significantly enhanced survival at lower inoculum densities (n = 6), accompanied by visible brown Mn oxides after 12 h, suggesting a protective role of these oxides. Significance (* p < 0.05) was assessed using the Wilcoxon test. ( d ) The relationship between survival and incremental Mn(II) oxidation rate in KT2440. Equation depicted the relationship, while R 2 indicated the linear correlation between survival and Mn(II) oxidation rate. In all regression analyses, the T-test yielded p -values less than 0.05.
Daptomycin, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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site a 30 30 0 30 0 30  (ATCC)
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ATCC site a 30 30 0 30 0 30
( a ) Colonies without MnCl₂ exhibited rapid expansion and increased margin irregularity, highlighting dynamic interactions within the microbial community. ( b ) Mn(II) oxidation rates by KT2440, assessed across various inoculum densities in co-culture with <t>PAO1</t> and in monoculture (n = 3), showed significant differences at lower densities. ( c ) Following MnCl₂ addition, KT2440 demonstrated significantly enhanced survival at lower inoculum densities (n = 6), accompanied by visible brown Mn oxides after 12 h, suggesting a protective role of these oxides. Significance (* p < 0.05) was assessed using the Wilcoxon test. ( d ) The relationship between survival and incremental Mn(II) oxidation rate in KT2440. Equation depicted the relationship, while R 2 indicated the linear correlation between survival and Mn(II) oxidation rate. In all regression analyses, the T-test yielded p -values less than 0.05.
Site A 30 30 0 30 0 30, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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site b site c kpc klebsiella pneumoniae ar bank  (ATCC)
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ATCC site b site c kpc klebsiella pneumoniae ar bank
( a ) Colonies without MnCl₂ exhibited rapid expansion and increased margin irregularity, highlighting dynamic interactions within the microbial community. ( b ) Mn(II) oxidation rates by KT2440, assessed across various inoculum densities in co-culture with <t>PAO1</t> and in monoculture (n = 3), showed significant differences at lower densities. ( c ) Following MnCl₂ addition, KT2440 demonstrated significantly enhanced survival at lower inoculum densities (n = 6), accompanied by visible brown Mn oxides after 12 h, suggesting a protective role of these oxides. Significance (* p < 0.05) was assessed using the Wilcoxon test. ( d ) The relationship between survival and incremental Mn(II) oxidation rate in KT2440. Equation depicted the relationship, while R 2 indicated the linear correlation between survival and Mn(II) oxidation rate. In all regression analyses, the T-test yielded p -values less than 0.05.
Site B Site C Kpc Klebsiella Pneumoniae Ar Bank, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( a ) Colonies without MnCl₂ exhibited rapid expansion and increased margin irregularity, highlighting dynamic interactions within the microbial community. ( b ) Mn(II) oxidation rates by KT2440, assessed across various inoculum densities in co-culture with PAO1 and in monoculture (n = 3), showed significant differences at lower densities. ( c ) Following MnCl₂ addition, KT2440 demonstrated significantly enhanced survival at lower inoculum densities (n = 6), accompanied by visible brown Mn oxides after 12 h, suggesting a protective role of these oxides. Significance (* p < 0.05) was assessed using the Wilcoxon test. ( d ) The relationship between survival and incremental Mn(II) oxidation rate in KT2440. Equation depicted the relationship, while R 2 indicated the linear correlation between survival and Mn(II) oxidation rate. In all regression analyses, the T-test yielded p -values less than 0.05.

Journal: bioRxiv

Article Title: Physiological Significance of Bacterial Mn(II) Oxidation

doi: 10.1101/2025.11.24.690301

Figure Lengend Snippet: ( a ) Colonies without MnCl₂ exhibited rapid expansion and increased margin irregularity, highlighting dynamic interactions within the microbial community. ( b ) Mn(II) oxidation rates by KT2440, assessed across various inoculum densities in co-culture with PAO1 and in monoculture (n = 3), showed significant differences at lower densities. ( c ) Following MnCl₂ addition, KT2440 demonstrated significantly enhanced survival at lower inoculum densities (n = 6), accompanied by visible brown Mn oxides after 12 h, suggesting a protective role of these oxides. Significance (* p < 0.05) was assessed using the Wilcoxon test. ( d ) The relationship between survival and incremental Mn(II) oxidation rate in KT2440. Equation depicted the relationship, while R 2 indicated the linear correlation between survival and Mn(II) oxidation rate. In all regression analyses, the T-test yielded p -values less than 0.05.

Article Snippet: Two Pseudomonas strains were used in the study, P. aeruginosa PAO1 (attacker, accession no. NBRC 100989) and P. putida KT2440 (Mn(II)-oxidizing bacteria, ATCC 11996).

Techniques: Co-Culture Assay

We co-cultured PAO1 (expressing GFP) and KT2440 (expressing mCherry, RFP) cells: ( a ) without MnCl₂ and ( b ) with MnCl₂ after 24 h of growth. In the presence of MnCl₂, especially at lower densities, KT2440 mCh cells were encapsulated in Mn oxide patches distributed throughout the colony center. ( c ) This encapsulation led to a significant increase in KT2440 mCh survival across various densities (n = 6). Statistical significance (* p < 0.05) was determined using the Wilcoxon test.

Journal: bioRxiv

Article Title: Physiological Significance of Bacterial Mn(II) Oxidation

doi: 10.1101/2025.11.24.690301

Figure Lengend Snippet: We co-cultured PAO1 (expressing GFP) and KT2440 (expressing mCherry, RFP) cells: ( a ) without MnCl₂ and ( b ) with MnCl₂ after 24 h of growth. In the presence of MnCl₂, especially at lower densities, KT2440 mCh cells were encapsulated in Mn oxide patches distributed throughout the colony center. ( c ) This encapsulation led to a significant increase in KT2440 mCh survival across various densities (n = 6). Statistical significance (* p < 0.05) was determined using the Wilcoxon test.

Article Snippet: Two Pseudomonas strains were used in the study, P. aeruginosa PAO1 (attacker, accession no. NBRC 100989) and P. putida KT2440 (Mn(II)-oxidizing bacteria, ATCC 11996).

Techniques: Cell Culture, Expressing, Encapsulation